4-HO-DET is a novel synthetic tryptamine and is the N,N-diethyl structural homologue of psilocin.
4-HO-DET has been isolated from Psilocybe cubensis grown from mycelial substrate supplemented with N,N-DET (N,N-diethyltryptamine) hydrochloride.  This was discovered by Jochen Gartz, a colleague of Alexander Shulgin. Not only that, but 4-PO-DET (4-phosphoryloxy-N,N-diethyltryptamine) was isolated as well, and the mushrooms yielded up to 3.3% alkaloid content by dried weight (whereas the typicla magic mushroom only contains 1-2% psilocin/psilocybin by dried weight, on average). The ideal saturation was found to be 0.25 mmol per 13.5 grams of mycelial substrate. Spores for Psilocybe cubensis were inoculated into the substrate.
Further research from Gartz also found that adding NMT to the substrate yielded mushrooms with almost exclusively 4-HO-NMT (4-hydroxy-N-methyltryptamine) and baeocystin (4-PO-NMT, 4-phoshporyloxy-N-methyltryptamine). Additionally, adding DPT to the substrate yielded mushrooms with 4-HO-DPT/4-PO-DPT, and adding DiPT to the substrate yielded mushrooms with 4-HO-DiPT/4-PO-DiPT . It was determined that the enzymes responsible for the biosynthesis of psilocin/psilocybin (the route typically being tryptophan->tryptamine->NMT/DMT->4-HO-NMT/psilocin->baeocystin/psilocybin) can recognize a potentially wide variety of tryptamines as enzymatic substrate, including synthetic tryptamines, and produce biosynthetic psilocin analogs as metabolites.
It is NOT intended for human consumption, and is strictly for laboratory reagent or forensic analysis purposes ONLY. Available as the fumarate salt.
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 - http://countyourculture.com/2012/02/17/biosynthesis-4-substituted-tryptamine-derivatives/
 - http://www.cognitiveliberty.org/shulgin/blg/2005/12/4-hydroxy-5-methoxy-nn_07.html